Cats bite and scratch: Where is the line from play to aggression?

Ear pressing, pupil dilation, and tail twitching are the three signals before upgrading.
There is a clear warning chain in a cat's body language: first the tail twitches quickly, then the ears press sideways, then the pupils dilate and the whiskers tilt forward. If you continue to play when these three signals appear, getting bitten is the inevitable result.
The difference between a playful bite and an aggressive bite is intent. Game bites are usually accompanied by a relaxed body and unretracted claws, and are an extension of the hunting sequence; attack bites include hissing, body arching, and active pursuit, and are often triggered by pain, fear, or territorial conflicts.
The core of correcting game bites is to "stop interaction": if bitten, immediately stop, withdraw your hand, turn around and leave, and ignore it for ten minutes. Replace hands with toys and let the hunting sequence fall on the stuffed mouse, not your wrist.
Never slap or spray water. Punishment will cause the cat to associate the attack with the approach of the person. In the long run, the cat will upgrade from a game bite to a defensive attack, and the difficulty of handling will increase exponentially.
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